VALIDATION

 

In analyses of 10 mock communities each containing about 10 to 15 taxa, there was good correlation between QIIME and HOMINGS. Each method detected the appropriate taxon as shown in the figure below (click on figure to enlarge).

 

Probes were validated against 16S rRNA gene sequences in several databases including HOMD, GreenGenes, GenBank and the Ribosomal Database Project. In addition, sequences were validated in silico against several large datasets from our own studies (Dewhirst et al. 2010) in which there were typically multiple to hundreds of representatives for each taxon.