SAMPLE PREPARATION
Recommended DNA isolation protocol. The following procedure was suitable for isolation of DNA from hard-to-lyse species, such as S. mutans, Staphylococcus spp., Actinomyces spp., and Scardovia spp. This procedure worked well for samples from all oral sites including saliva, supragingival or subgingival plaque, buccal epithelial cells, scrapings from tongue surfaces, palatal and throat swabs, and denture surfaces.
The modified Protocol uses Ready-Lyse™ Lysozyme Solution for overnight incubation and MasterPure DNA Purification Kit.
Other adequate lysis methods are available. However, note that some protocols may not be sufficient for DNA isolation from the hard-to-lyse oral species. Clearly, if there is not adequate lysis, then those species not lysed will not be detected using molecular means of identification.